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Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
IBTK antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).

IBTK Antibody

CATALOG NUMBER: 55-375

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This IBTK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-613 amino acids from the Central region of human IBTK.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
151 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IBTK
Additional Names:
Inhibitor of Bruton tyrosine kinase, IBtk, IBTK, BTKI, KIAA1417
Protein Accession Number:
Q9P2D0
Protein Gi Number:
134034141
Ncbi Gene Id Number:
25998
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene binds to Bruton'styrosine kinase (BTK) and downregulates BTK's kinase activity. Inaddition, the encoded protein disrupts BTK-mediated calciummobilization and negatively regulates the activation of nuclearfactor-kappa-B-driven transcription.
Background Reference 1:
Fiume, G., et al. Comput Biol Chem 33(6):434-439(2009)
Background Reference 2:
Spatuzza, C., et al. Nucleic Acids Res. 36(13):4402-4416(2008)
Background Reference 3:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-375

List Size:
400 ul

List Price:

$495.00

Datasheet
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