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Western blot analysis of lysates from Mouse lung using ICAM-1/CD54 Rabbit mAb at 1:10000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.
Western blot analysis of lysates from Mouse lung using ICAM-1/CD54 Rabbit mAb at 1:10000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.

ICAM-1/CD54 Rabbit Recombinant Antibody (82-150)

CATALOG NUMBER: 82-150

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB,1:2000 - 1:10000, IHC-P,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Mouse lung
Predicted Molecular Weight:
59kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Icam1
Additional Names:
CD54; Ly-47; Icam-1; MALA-2; ICAM-1/CD54
Protein Accession Number:
P13597
Ncbi Gene Id Number:
15894
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an integral membrane protein that binds leukocyte adhesion protein LFA-1. It participates in the innate immune response.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-150

List Size:
100 uL

List Price:

$477.00

Datasheet
Shipping Info

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