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Western blot analysis of ICAM1 using rabbit polyclonal ICAM1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ICAM1 gene (Lane 2).
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ICAM1 using rabbit polyclonal ICAM1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ICAM1 gene (Lane 2).

ICAM1 Antibody

CATALOG NUMBER: 63-572

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ICAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 488-514 amino acids from the C-terminal region of human ICAM1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
58 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ICAM1
Additional Names:
Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1
Protein Accession Number:
P05362
Protein Gi Number:
68067956
Ncbi Gene Id Number:
3383
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ICAM1 is a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
Background Reference 1:
Denkers,I.A., et.al., Leuk. Res. 16 (5), 469-474 (1992)
Background Reference 2:
Rossler,K., et.al., J. Neurosci. Res. 31 (2), 365-374 (1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-572

List Size:
400 ul

List Price:

$495.00

Datasheet
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