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Immunohistochemistry staining of ICAM1 in spleen tissue using ICAM1 monoclonal Antibody.
Immunohistochemistry staining of ICAM1 in spleen tissue using ICAM1 monoclonal Antibody.

ICAM1 Antibody, Monoclonal

CATALOG NUMBER: 51-076

Clonality:
Monoclonal
Tested Applications:
Flow, IHC, IP
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
ICAM1 monoclonal antibody was raised against Human monocytes (Human).
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC, IP
Application Note:
ICAM1 antibody can be used in Western Blot starting at 0.5 - 1 μg/mL, and immunohistochemistry starting at 5 μg/mL. Flow Cytometry 1:50-1:100 Immunohistochemistry (frozen)- 1:50-1:100, Immunohistochemistry (FFPE), Immunoprecipitation. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Immunohistochemistry Recommended positive control tissue: Human Tonsil. Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Clone 3H1547 blocks CD54 function. Removal of sodium azide is recommended prior to use in functional assays.
Specificity:
Species Crossreactivity: Clone 3H1547 is reported to crossreact with porcine endothelium.
Purification:
Protein G Column
Clonality:
Monoclonal
Isotype:
IgG1
Physical State:
Liquid
Buffer:
PBS, pH 7.4, 0.09% sodium azide
Concentration:
1 mg/ml
Storage Conditions:
Store ICAM1 antibody at 4 °C or -20 °C. As with all antibodies avoid freeze/thaw cycles.
Ncbi Official Symbol:
ICAM1
Additional Names:
ICAM1, BB2, CD54 antigen, CD54, ICAM-1, p3.58
Protein Accession Number:
P05362
Protein Gi Number:
68067956
Ncbi Gene Id Number:
3383
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ICAM1 Antibody, Monoclonal

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

51-076

List Size:
0.05 mg

List Price:

$480.00

Out of stock

Datasheet
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