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45-758 (2.5ug/ml) staining of paraffin embedded Human Brain (Cerebral Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-758 (2.5ug/ml) staining of paraffin embedded Human Brain (Cerebral Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

ICAM5 Precursor Antibody

CATALOG NUMBER: 45-758

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-ESPAEGEVFAIQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 200-250kDa band in lysates of Human Brain (Frontal Cortex and Hippocampus) and Mouse Brain after 0.05 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.2kDa according to NP_003250.2. The 200-250kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Brain (Cerebral Cortex) shows axon/dendrite staining leaving cell bodies relatively unstained. Recommended concentration, 2-4ug/ml.
Positive Control 1:
Cat. No. XBL-10106 - Human Cerebral Cortex Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ICAM5
Additional Names:
ICAM5, intercellular adhesion molecule 5, telencephalin , TLCN, TLN , intercellular adhesion molecule 5, telencephalin
Protein Accession Number:
NP_003250.3
Protein Gi Number:
12545404
Ncbi Gene Id Number:
7087
Background Reference 1:
Yoshihara Y, Oka S, Nemoto Y, Watanabe Y, Nagata S, Kagamiyama H, Mori K. An ICAM-related neuronal glycoprotein, telencephalin, with brain segment-specific expression. Neuron. 1994 Mar;12(3):541-53.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-758

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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