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43-118 (2ug/ml) staining of nuclear Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-118 (2ug/ml) staining of nuclear Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ICBP90 / UHRF1 Antibody

CATALOG NUMBER: 43-118

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-LNQLFPGYGNGR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 100kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 91.1kDa according to NP_037414.3). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Hopfner, Cancer Res. 2000 Jan 1;60(1):121-8. PMID: 10646863). Recommended concentration: 1-3ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms.
Predicted Molecular Weight:
Approx 100kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ICBP90 / UHRF1
Additional Names:
UHRF1, ubiquitin-like, containing PHD and RING finger domains, 1, Np95, ICBP90, FLJ21925, transcription factor ICBP90, RNF106, MGC138707, RING finger protein 106, inverted CCAAT box-binding protein of 90 kDa, nuclear zinc finger protein Np95, transcription factor ICBP90, ubiquitin-like with PHD and ring finger domains 1, hNP95, E3 ubiquitin-protein ligase UHRF1,
Protein Accession Number:
NP_001041666.1,NP_037414.3
Protein Gi Number:
115430235
Ncbi Gene Id Number:
29128
Background Reference 1:
Hopfner R, Mousli M, Jeltsch JM, Voulgaris A, Lutz Y, Marin C, Bellocq JP, Oudet P, Bronner C. ICBP90, a novel human CCAAT binding protein, involved in the regulation of topoisomerase IIalpha expression.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-118

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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