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Western blot analysis in U251 cell line lysates (35ug/lane).
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in U251 cell line lysates (35ug/lane).

ICEF1 Antibody

CATALOG NUMBER: 55-408

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This ICEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 313-341 amino acids from the C-terminal region of human ICEF1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
49 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IPCEF1
Additional Names:
Interactor protein for cytohesin exchange factors 1, Phosphoinositide-binding protein PIP3-E, IPCEF1, KIAA0403
Protein Accession Number:
Q8WWN9
Protein Gi Number:
74751582
Ncbi Gene Id Number:
26034
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enhances the promotion of guanine-nucleotide exchange by PSCD2 on ARF6 in a concentration-dependent manner (By similarity).
Background Reference 1:
Bailey, S.D., et al. Diabetes Care (2010) In press :
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
White, D.T., et al. Mol. Biol. Cell 21(4):562-571(2010)
Background Reference 4:
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-408

List Size:
400 ul

List Price:

$495.00

Datasheet
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