Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Overlay histogram showing HepG2 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western blot analysis of lysates from HepG2, MCF-7, U-2OS cell line (from left to right), using ID1 Antibody at 1:1000 at each lane.
Western blot analysis in U251 cell line lysates (35ug/lane).
Western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ID1(PEI 1:1detected the ID1 protein (arrow).
ID1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of ID1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Overlay histogram showing HepG2 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

ID1 Antibody

CATALOG NUMBER: 56-010

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
This ID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the Central region of human ID1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
16 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ID1
Additional Names:
DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, bHLHb24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, ID1, BHLHB24, ID
Protein Accession Number:
P41134
Protein Gi Number:
21264450
Ncbi Gene Id Number:
3397
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a helix-loop-helix(HLH) protein that can form heterodimers with members of the basicHLH family of transcription factors. The encoded protein has no DNAbinding activity and therefore can inhibit the DNA binding andtranscriptional activation ability of basic HLH proteins with whichit interacts. This protein may play a role in cell growth,senescence, and differentiation. Two transcript variants encodingdifferent isoforms have been found for this gene. [provided byRefSeq].
Background Reference 1:
Qian, T., et al. Oncogene 29(43):5818-5827(2010)
Background Reference 2:
Hamajima, Y., et al. Cell Prolif. 43(5):457-463(2010)
Background Reference 3:
Yang, J., et al. Circ. Res. 107(2):252-262(2010)
Background Reference 4:
Phi, J.H., et al.Neurosurg Pediatr 5(6):608-614(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-010

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369