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Antibody used in WB on Human Thymus at 0.2-1 ug/ml.
Antibody used in WB on Human Thymus at 0.2-1 ug/ml.

ID2 Antibody

CATALOG NUMBER: 27-257

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ID2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ID2 antibody can be used for detection of ID2 by ELISA at 1:1562500. ID2 antibody can be used for detection of ID2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10426 - Fetal Thymus Tissue Lysate
Predicted Molecular Weight:
15 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ID2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ID2
Additional Names:
ID2, GIG8, ID2A, ID2H, MGC26389, bHLHb26
Protein Accession Number:
NP_002157
Protein Gi Number:
31982933
Ncbi Gene Id Number:
3398
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ID2 belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation.The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene has been identified for this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background Reference 1:
Yang, J., (2008) Circ. Res. 102 (10), 1212-1221.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

27-257

List Size:
100 ul

List Price:

$519.00

Datasheet
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