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45-760 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-760 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Ide Antibody

CATALOG NUMBER: 45-760

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-QQYNYDRDNIE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 110kDa band observed in Mouse Brain and Rat Brain lysates (calculated MW of 118kDa according to NP_112419.2). Recommended concentration: 1-3ug/ml.An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Ide
Additional Names:
Ide, insulin-degrading enzyme, 1300012G03Rik, 4833415K22Rik, AA675336, AI507533, INSULYSIN, insulinase, insulysin
Protein Accession Number:
NP_112419.2
Protein Gi Number:
121583922
Ncbi Gene Id Number:
15925(mouse);25700(rat);
Background Reference 1:
Zhao Z, Xiang Z, Haroutunian V, Buxbaum JD, Stetka B, Pasinetti GM. Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease. Neurobiol Aging. 2007 Jun;28(6):824-30. Epub 2006 Jun 12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-760

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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