Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A375 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: mouse liver lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of anti-IDE Antibody in A375 cell line lysates (35ug/lane)

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IDE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: A375 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: mouse liver lysate Lysates/proteins at 20 ug per lane.

IDE Antibody

CATALOG NUMBER: 57-818

Clonality:

Polyclonal

Tested Applications:

IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse

Homology:

Predicted species reactivity based on immunogen sequence: Rat, Bovine

Immunogen:

This IDE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 406-435 amino acids from the Central region of human IDE.

Conjugate:

Unconjugated

Tested Applications:

IHC-P, WB

Application Note:

For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:10~50

Predicted Molecular Weight:

118 kDa

Advanced Validation

Properties

Purification:

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

Clonality:

Polyclonal

Isotype:

Rabbit Ig

Physical State:

Liquid

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Storage Conditions:

Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

Ncbi Official Symbol:

IDE

Additional Names:

Insulin-degrading enzyme, Abeta-degrading protease, Insulin protease, Insulinase, Insulysin, IDE

Protein Accession Number:

P14735

Protein Gi Number:

215274252

Ncbi Gene Id Number:

3416

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

IDE belongs to a protease family responsible for intercellular peptide signalling. Though its role in the cellular processing of insulin has not yet been defined, insulin-degrading enzyme is thought to be involved in the termination of the insulin response.

Background Reference 1:

Vepsalainen,S.,J. Med. Genet. 44 (9), 606-608 (2007)

Background Reference 2:

Kim,M.,J. Biol. Chem. 282 (11), 7825-7832 (2007)

Background Reference 3:

Radulescu,R.T.,Int. J. Oncol. 30 (1), 73-80 (2007)

Background Reference 4:

Li,Q.,Cell 127 (2), 305-316 (2006)

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-818

List Size:

400 ul

List Price:

$495.00

Datasheet

Shipping Info

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IDE Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Homology:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Protein Gi Number:

Ncbi Gene Id Number:

User Note:

Background:

Background Reference 1:

Background Reference 2:

Background Reference 3:

Background Reference 4:

Disclaimer:

List Size:

Shipping Info

Customize your order

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