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Western blot analysis of IDH1 in HepG2 cell lysate with IDH1 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of IDH1 in HepG2 cell lysate with IDH1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

IDH1 Antibody

CATALOG NUMBER: 5819

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (100%), Bovine: (100%)
Immunogen:
IDH1 antibody was raised against a 13 amino acid synthetic peptide near the carboxy terminus of human IDH1.
The immunogen is located within the last 50 amino acids of IDH1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
IDH1 antibody can be used for detection of IDH1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Purification:
IDH1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IDH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IDH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IDH1
Additional Names:
IDH1 Antibody: IDH, IDP, IDCD, IDPC, PICD, HEL-216, HEL-S-26, Cytosolic NADP-isocitrate dehydrogenase, IDH
Protein Accession Number:
NP_005887
Protein Gi Number:
28178825
Ncbi Gene Id Number:
3417
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IDH1 Antibody: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. The presence of IDH1 in peroxisomes suggests it may play a role in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic IDH1 serves a significant role in cytoplasmic NADPH production. Defects in IDH1 are involved in the development of glioma.
Background Reference 1:
Geisbrecht BV and Gould SJ. The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase. J. Biol. Chem.1999; 274:30527-33.
Background Reference 2:
Xu X, Zhao J, Xu Z, et al. Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity. J. Biol. Chem.2004; 279:33946-57.
Background Reference 3:
Dang L, White DW, and Gross S. Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature2009; 462:739-44.
Background Reference 4:
Smeitink J. Metabolism, gliomas, and IDH1. N. Engl. J. Med.2010; 362:1144-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5819

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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