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42-704 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-704 (0.1ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-704 (3.75ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-704 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

IDH2 Antibody

CATALOG NUMBER: 42-704

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: CIHGLSNVKLNE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 48kDa band observed in Mouse, Rat and Pig Heart lysates (calculated MW of 50.9kDa according to Mouse NP_766599.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 2:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 3:
Cat. No. 1461 - Rat Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IDH2
Additional Names:
IDH2, isocitrate dehydrogenase 2 (NADP+), mitochondrial, ICD-M, IDH, IDHM, IDP, IDPM, mNADP-IDH, NADP+-specific ICDH, isocitrate dehydrogenase, mitochondrial, oxalosuccinate decarboxylase
Protein Accession Number:
NP_002159.2
Protein Gi Number:
28178832
Ncbi Gene Id Number:
3418

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-704

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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