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42-381 (0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-381 (0.1ug/ml) staining of Human (A), Mouse (B) and Rat (C) Spleen lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-381 (0.3ug/ml) staining of Pig Spleen (A) and Pig Skeletal Muscle (B) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-381 (3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-381 (0.1ug/ml) staining of Human Lymph Nodes lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

IDH3A Antibody

CATALOG NUMBER: 42-381

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: DFTEEICRRVKDLD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI, and 38kDa in Human, Mouse and Rat Spleen lysates and in Pig Spleen and Skeletal Muscle lysates (calculated MW of 39.6kDa according to NP_005521.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Positive Control 2:
Human Lymph Nodes
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IDH3A
Additional Names:
IDH3A, isocitrate dehydrogenase 3 (NAD+) alpha, H-IDH alpha, NAD(+)-specific ICDH subunit alpha, NAD(H)-specific isocitrate dehydrogenase alpha subunit, NAD+-specific ICDH, isocitrate dehydrogenase (NAD+) alpha chain, isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, isocitric dehydrogenase subunit alpha
Protein Accession Number:
NP_005521.1
Protein Gi Number:
5031777
Ncbi Gene Id Number:
3419

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-381

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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