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42-380 (0.3ug/ml) staining of Human Skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-380 (2ug/ml) staining of Mouse Heart (A) and Mouse Muscle (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-380 (0.1ug/ml) staining of Pig Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-380 (0.3ug/ml) staining of Human Skeletal muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

IDH3B Antibody

CATALOG NUMBER: 42-380

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-HAASRSQAEDVRVE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 39kDa band observed in Mouse, Rat and Pig Heart lysates and in Human and Mouse Skeletal Muscle lysates (calculated MW of 41.9kDa according to NP_777280.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
This antibody is expected to recognize isoform a (NP_008830.2) and isoform b (NP_777280.1).
Positive Control 1:
Cat. No. 1301 - Human Heart Tissue Lysate
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Positive Control 2:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate
Positive Control 3:
Cat. No. 1461 - Rat Heart Tissue Lysate
Cat. No. 1467 - Rat Skeletal Muscle Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IDH3B
Additional Names:
IDH3B, isocitrate dehydrogenase 3 (NAD+) beta, FLJ11043, H-IDHB, MGC903, RP46, NAD(+)-specific ICDH subunit beta, NAD+-specific ICDH, NAD+-specific isocitrate dehydrogenase b subunit, NAD+-specific isocitrate dehydrogenase beta, isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, beta subunit, isocitric dehydrogenase subunit beta
Protein Accession Number:
NP_008830.2,NP_777280.1
Protein Gi Number:
28178821
Ncbi Gene Id Number:
3420

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-380

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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