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Immunohistochemical analysis of paraffin-embedded H.brain section using IDH3B Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from Hela cell line and human placenta tissue lysate (from left to right), using IDH3B Antibody at 1:1000 at each lane.
Immunohistochemical analysis of paraffin-embedded H.brain section using IDH3B Antibody (N-term). Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

IDH3B Antibody

CATALOG NUMBER: 61-033

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This IDH3B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the N-terminal region of human IDH3B.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IDH3B
Additional Names:
Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial, Isocitric dehydrogenase subunit beta, NAD(+)-specific ICDH subunit beta, IDH3B
Protein Accession Number:
O43837
Ncbi Gene Id Number:
3420
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background Reference 1:
Kim Y.-O.,et al.J. Biol. Chem. 274:36866-36875(1999).
Background Reference 2:
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Background Reference 3:
Deloukas P.,et al.Nature 414:865-871(2001).
Background Reference 4:
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-033

List Size:
400 ul

List Price:

$495.00

Datasheet
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