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42-379 (0.5ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-379 (1ug/ml) staining of Rat (A) and Pig (B) Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-379 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-379 (0.5ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

IDH3G Antibody

CATALOG NUMBER: 42-379

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-DHLKLHSYATSIRK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38kDa band observed in lysates of cell line MCF7 and of Rat and Pig Heart (calculated MW of 41.5kDa according to NP_777358.1). Recommended concentration: 0.5-2ug/ml.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_004126.1; NP_777358.1).
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Positive Control 2:
Cat. No. 1301 - Human Heart Tissue Lysate
Positive Control 3:
Cat. No. 1401 - Mouse Heart Tissue Lysate
Positive Control 4:
Cat. No. 1461 - Rat Heart Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IDH3G
Additional Names:
IDH3G, isocitrate dehydrogenase 3 (NAD+) gamma, H-IDHG, IDH-gamma, NAD (H)-specific isocitrate dehydrogenase gamma subunit, NAD(+)-specific ICDH subunit gamma, NAD+-specific ICDH, OTTHUMP00000025984, OTTHUMP00000025987, OTTHUMP00000025988, isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit, isocitric dehydrogenase subunit gamma
Protein Accession Number:
NP_004126.1,NP_777358.1
Protein Gi Number:
4758582
Ncbi Gene Id Number:
3421

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-379

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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