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Western blot analysis of lysate from human placenta tissue lysate, using IDH3G Antibody at 1:1000 at each lane.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from human placenta tissue lysate, using IDH3G Antibody at 1:1000 at each lane.

IDH3G Antibody

CATALOG NUMBER: 64-122

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey
Immunogen:
This IDH3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the C-terminal region of human IDH3G.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IDH3G
Additional Names:
Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, Isocitric dehydrogenase subunit gamma, NAD(+)-specific ICDH subunit gamma, IDH3G
Protein Accession Number:
P51553
Protein Gi Number:
1708404
Ncbi Gene Id Number:
3421
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia.
Background Reference 1:
Bzymek, K.P., et al. Biochemistry 46(18):5391-5397(2007)
Background Reference 2:
Soundar, S., et al. J. Biol. Chem. 281(30):21073-21081(2006)
Background Reference 3:
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
Background Reference 4:
Weiss, C., et al. Biochemistry 39(7):1807-1816(2000)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-122

List Size:
400 ul

List Price:

$495.00

Datasheet
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