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IDO Antibody

CATALOG NUMBER: 36-231

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Recombinant mouse IDO.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IHC, WB
Application Note:
ELISA: (direct and indirect: 1:2,000-1:10,000). Immunocytochemistry: (1:500-1:2,000). Immunohistochemistry: (paraffin sections (1:200-1:500)). Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
Specificity:
Recognizes mouse IDO. Detects a band of ~45kDa by Western blot.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Storage Conditions:
Stable for at least 6 months after receipt when stored at -20°C.
Ncbi Official Symbol:
Ido1
Additional Names:
Indoleamine 2,3-dioxygenase; INDO
Protein Accession Number:
123949
Protein Gi Number:
P28776
Ncbi Gene Id Number:
15930
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

36-231

List Size:
100 ug

List Price:

$463.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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