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IDO Antibody [ID 177]

CATALOG NUMBER: 36-036

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Recombinant human IDO.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA: (direct or indirect: 1:2,000-1:10,000). Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Specificity:
Recognizes human IDO. Detects a band of ~45kDa by Western blot. Other species not tested.
Clonality:
Monoclonal
Isotype:
IgG1, kappa
Physical State:
Liquid
Buffer:
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
Concentration:
1 mg/ml
Storage Conditions:
Stable for at least 1 year after receipt when stored at -20°C.
Ncbi Official Symbol:
IDO1
Additional Names:
Indoleamine 2,3-dioxygenase; INDO
Protein Accession Number:
4504577
Protein Gi Number:
NP_002155.1
Ncbi Gene Id Number:
3620
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-gamma induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

36-036

List Size:
100 ug

List Price:

$408.00

Datasheet
Shipping Info

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