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Western blot analysis of IEX-1 in mouse skin tissue lysate with IEX-1 antibody at (A) 1 and (B) 2 μg/mL..
Immunohistochemistry of IEX-1 in human liver tissue with IEX-1 antibody at 2.5 μg/mL.
Immunofluorescence of IEX-1 in Human Brain cells with IEX-1 antibody at 20 μg/mL.
Western blot analysis of IEX-1 in mouse skin tissue lysate with IEX-1 antibody at (A) 1 and (B) 2 μg/mL..
Trial Size

IEX-1 Antibody

CATALOG NUMBER: 4247

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
IEX1 antibody was raised against a 24 amino acid synthetic peptide near the center of human IEX1.
The immunogen is located within amino acids 40 - 90 of IEX-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
IEX1 antibody can be used for detection of IEX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 3:
Cat. No. 1419 - Mouse Skin Tissue Lysate
Positive Control 4:
Cat. No. 10-201 - Human Liver Tissue Slide
Predicted Molecular Weight:
Predicted: 17 kDa
Observed: 17 kDa
Purification:
IEX-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IEX-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IEX-1 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IER3
Additional Names:
IEX-1 Antibody: DIF2, IEX1, PRG1, DIF-2, GLY96, IEX-1, IEX-1L, DIF2, Radiation-inducible immediate-early gene IEX-1, Differentiation-dependent gene 2 protein, Protein DIF-2
Protein Accession Number:
P46695
Protein Gi Number:
3123229
Ncbi Gene Id Number:
8870
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IEX-1 Antibody: IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-α. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-κB/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.
Background Reference 1:
Kondratyev AD, Chung K-N and Jung MO. Identification and characterization of a radiation-inducible glycosylated human early-response gene. Cancer Res.1996; 56:1498-502.
Background Reference 2:
Feldmann KA, Piddelkow MR, Roche PC, et al. Expression of an immediate early gene, IEX-1, in human tissues. Histochem. Cell Biol.2001; 115:489-97.
Background Reference 3:
Wu MX, Zhaohui A, Prasad KVS, et al. IEX-1L, an apoptosis inhibitor involved in NFkappaB mediated cell survival. Science1998; 281:998-1001.
Background Reference 4:
Arlt A, Grobe O, Sieke A, et al. Expression of the NF-kappa B target gene IEX-1 (p22/PRG1) does not prevent cell death but instead triggers apoptosis in Hela cells. Oncogene2001; 20:69-76.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4247

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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