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<strong>Figure 1 WB Validation in Human and Rat Cell Lines </strong><br>Loading: 15 μg of lysate per lane. Antibodies: IFI16 9641, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysates per lane. Antibodies: IFI16 9641, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Western Blot Validation in Rat Testis</strong><br>Loading: 15 μg of lysate per lane. Antibodies: IFI16 9641, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 4 Immunofluorescence Validation of IFI16 in Human Molt4 Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Molt4 cells labeling IFI16 with 9641 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunohistochemistry Validation of IFI16 in Human Testis</strong><br>Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-IFI16 antibody (9641) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunohistochemistry Validation of IFI16 in Mouse Liver</strong><br>Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-IFI16 antibody (9641) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of IFI16 in Rat Testis</strong><br>Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-IFI16 antibody (9641) at 1 μg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 WB Validation in Human and Rat Cell Lines
Loading: 15 μg of lysate per lane. Antibodies: IFI16 9641, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.
New
Trial Size

IFI16 (CT) Antibody

CATALOG NUMBER: 9641

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Gorilla (100%), chimpanzee (100%)
Immunogen:
Anti-IFI16 antibody (9641) was raised against a peptide corresponding to 16 amino acids near the carboxyl terminus of human IFI16.

The immunogen is located within the last 50 amino acids of IFI16.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC, WB
Application Note:
WB: 2 μg/mL; IF: 20 μg/mL; IHC: 1 μg/mL.

Antibody validated: Western Blot in human, mouse, and rat samples; Immunofluorescence in human samples; Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-06 - Molt4 Cell Slide
Predicted Molecular Weight:
Predicted: 88kD

Observed: 88 kD
Isoforms:
Human IFI16 has 4 isoforms, including isoform 1 (785aa, 8.3kD), isoform 2 (729aa, 82.1kD), isoform 3(673aa, 75.9kD), and isoform 4 (729aa, 82.6kD)
Purification:
IFI16 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IFI16 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IFI16 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IFI16
Additional Names:
IFI16 Antibody: Gamma-interferon-inducible protein 16, Ifi-16, Interferon-inducible myeloid differentiation transcriptional activator, IFNGIP1
Protein Accession Number:
Q16666.3
Protein Gi Number:
118572657
Ncbi Gene Id Number:
3428
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IFI16 Antibody: IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer. Increased expression of IFI16 predicts adverse prognosis in multiple myeloma. IFI16 isoforms with cytoplasmic and nuclear locations play differential roles in recognizing invaded DNA viruses. IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection. Nuclear sensor IFI16 inhibits the function of Hepatitis B virus covalently closed circular DNA by integrating innate immune activation and epigenetic suppression.
Background Reference 1:
Ka et al. Cell Rep. 2021; 37(12):110138
Background Reference 2:
Huang et al. Pharmacogenomics J. 2021; 21(4):520-532
Background Reference 3:
Li et al. J Immunol. 2021; 207(11):2699-2709
Background Reference 4:
Jiang et al. Nat Microbiol. 2021; 6(7):932-945
Background Reference 5:
Yang et al. Hepatology 2020; 71(4):1154-1169

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9641

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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