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To detect Human IFN-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IFN-beta (38-219) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-beta.
To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Human IFN-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human IFN-beta (38-219) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-beta.

IFN-beta Antibody

CATALOG NUMBER: 38-287

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
E.coli derived Recombinant Human IFN-β
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect Human IFN-beta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Polyclonal Biotinylated Anti-Human IFN-beta (38-219) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human IFN-beta.
Western Blot:
: To detect Human IFN-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Human IFN-beta (5.00μg/mL), a concentration of 2.5 μg/mL of this antibody is required.
Purification:
IFN-beta specific antibody was purified by affinity chromatography employing immobilized Human IFN-beta matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IFNB1
Additional Names:
IFB, IFF, IFNB, IFB, Interferon beta, Fibroblast interferon, IFN-beta
Protein Accession Number:
P01574
Protein Gi Number:
124469
Ncbi Gene Id Number:
3456
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-287

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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