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<strong>Figure 1 Western Blot Validation in Mouse A20 Cell Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IFN-beta 4243 (Lane 1: 5 μg/mL and Lane 2: 10 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IFN-beta 4243 (4 μg/mL), IFN-beta 64-239, (5 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Multiple Tissues and Recombinant Protein</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IFN-beta 4243 (4 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of IFN-beta in Human Liver Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody (4243) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunofluorescence Validation of IFN-beta in Human Liver Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human liver cells labeling IFN-beta with 4243 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
<strong>Figure 6 Immunohistochemistry Validation of IFN-beta in Human Liver Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IFN-beta antibody (4243) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunofluorescence Validation of IFN-beta in Mouse Liver Tissue</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse liver tissue labeling IFN-beta with 4243 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 Western Blot Validation in Mouse A20 Cell Lysate
Loading: 15 μg of lysates per lane.Antibodies: IFN-beta 4243 (Lane 1: 5 μg/mL and Lane 2: 10 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
Trial Size

IFN-beta Antibody

CATALOG NUMBER: 4243

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-IFN-beta antibody (4243) was raised against a peptide corresponding to 17 amino acids near the center of human IFN-beta.
The immunogen is located within amino acids 110-160 of IFN-beta.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WB: 4-10 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 3:
Cat. No. 10-201 - Human Liver Tissue Slide
Predicted Molecular Weight:
Predicted: 22kD
Observed: 22kD.
Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar IFN-beta expression profile in human and mouse cell lines detected by two independent anti-IFN-beta antibodies that recognize different epitopes, 4243 against central domain and 64-239 against human recombinant protein.  IFN-beta proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Recombinant Protein Test (Figure 3): Anti-IFN-beta antibodies (4243) detected human IFN-beta recombinant protein.

Isoforms:
Human IFN-beta has one (187aa, 22kD). Mouse IFN-beta has one isoform (182aa, 22kD) and Rat IFN-beta also has one isoform (184aa, 22kD). 4243 can detect human isoform, and possibly detect mouse and rat.
Purification:
IFN-beta Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IFN-beta Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IFN-beta antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IFNB1
Additional Names:
IFN-beta Antibody: IFB, IFF, IFNB, IFB, Interferon beta, Fibroblast interferon, IFN-beta
Protein Accession Number:
AAC41702
Protein Gi Number:
184623
Ncbi Gene Id Number:
3456
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IFN-beta Antibody: Type I Interferons (IFN-alpha/beta) are produced primarily in response to viral infection by "Natural IFN-producing cells" (IPCs) as part of the host immune response and can also inhibit the development of tumors. IFN-beta binding by its receptor results in the activation of the tyrosine kinases Jak1 and Tyk2 and phosphorylation of members of the STAT family of transcription factors, leading to the transcription and expression of the immune response genes. More recently, several members of the toll-like receptor (TLR) family were found to stimulate the production IFN-beta. IFN-beta is currently used clinically for treatment of tumors, infections and multiple sclerosis.
Background Reference 1:
Gresser. J. Leuk. Biol.1997; 61:567-74.
Background Reference 2:
Colonna. Eur. J. Immunol.2007; 37:306-9.
Background Reference 3:
Kock et al. Ann. Neurol.2007; epub.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4243

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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