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To detect Rat IFN-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat IFN-gamma (XP-5157Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-gamma.
To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat IFN-gamma by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat IFN-gamma (XP-5157Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-gamma.

IFN-gamma Antibody

CATALOG NUMBER: XP-5157

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Goat
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Rat
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rIFN-gamma (rat Interferon-gamma).
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of rIFN-gamma (0.3 ng/mL), a concentration of 0.006 - 0.01 μg/mL of this antibody is required.
ELISA:
To detect rIFN-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rIFN-gamma.
Sandwich:
To detect Rat IFN-γ by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IFN-γ (XP-5157Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-γ.
Western Blot:
To detect rIFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rIFN-gamma is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-rIFN-gamma specific antibody was purified by affinity chromatography employing immobilized rIFN-gamma matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IFN-gamma antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Ifng
Additional Names:
IFNG2Interferon gammaIFN-gamma
Protein Accession Number:
P01581
Protein Gi Number:
124482
Ncbi Gene Id Number:
25712
User Note:
Centrifuge vial prior to opening.
Background:
IFN gamma receptor beta is part of the receptor for interferon gamma. This class II cytokine receptor pairs with CDw119 to form the IFN gamma receptor and is an integral part of the IFN gamma signal transduction pathway. CDw119 serves as the IFN gamma binding chain and associates with the IFN gamma beta chain which is required for receptor signaling. The extracellular portion of both the IFN gamma receptor alpha and beta chains must be species matched. The IFN gamma receptor beta chain is expressed on T and B cells, NK cells, monocytes/ macrophages, and fibroblasts. Binding of IFN gamma induces receptor dimerization, internalization, Jak1 and Jak2 protein kinase activation and, ultimately, STAT1 activation. It is also likely to interact with GAF. IFN gamma initiates and regulates a variety of immune responses and is required for signal transduction. Contains 2 fibronectin type III domains. Defects in IFN gamma Receptor beta are a cause of mendelian susceptibility to mycobacterial disease (MSMD), a rare condition that confers predisposition to illness caused by several mycobacteria strains

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5157

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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