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Western blot analysis of extracts of various cell lines, using IFNA1 antibody (13-128) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of A549 cells using IFNA1 antibody (13-128). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using IFNA1 antibody (13-128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

IFNA1 Antibody

CATALOG NUMBER: 13-128

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-155 of human IFNA1 (NP_076918.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:100 - 1:200
Positive Control 1:
HL-60
Positive Control 2:
Mouse liver
Positive Control 3:
Rat liver
Predicted Molecular Weight:
Observed: 21kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IFNA1
Additional Names:
IFL, IFN, IFN-ALPHA, IFN-alphaD3, IFNA@, IFNA1, interferon alpha-1/13, IFN-alpha 1b, IFN-alpha-1/13, interferon alpha 1b, interferon alpha-D, interferon-alpha1, leIF D
Ncbi Gene Id Number:
3439
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-128

List Size:
100 uL

List Price:

$436.00

Datasheet
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