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Western blot analysis of extracts of various cell lines, using IFNAR2 antibody (18-046) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using IFNAR2 antibody (18-046). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using IFNAR2 antibody (18-046) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

IFNAR2 Antibody

CATALOG NUMBER: 18-046

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-243 of human IFNAR2 (NP_997467.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
MCF7
Positive Control 2:
Raji
Positive Control 3:
HepG2
Positive Control 4:
THP-1
Positive Control 5:
SW480
Positive Control 6:
A-549
Predicted Molecular Weight:
Observed: 58kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IFNAR2
Additional Names:
IFNAR2, interferon (alpha, beta and omega) receptor 2, IFN-R, IFN-alpha-REC, IFNABR, IFNARB
Ncbi Gene Id Number:
3455
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-046

List Size:
100 uL

List Price:

$436.00

Datasheet
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