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Western blot analysis using IFN - gamma monoclonal antibody against truncated IFN - gamma recombinant protein.
Western blot analysis using IFN - gamma monoclonal antibody against truncated IFN - gamma recombinant protein.

IFNgamma Antibody [3F1E3]

CATALOG NUMBER: 32-173

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant human IFN-γ expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:500 - 1,000
ELISA:Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
IFN-gamma monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IFNG
Additional Names:
Immune interferon, IFN-gamma, IFG, IFI
Protein Accession Number:
P01579
Protein Gi Number:
124479
Ncbi Gene Id Number:
3458
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Interferon-g (IFN-γ) is a pro-inflammatory cytokine that is central in host resistance to infection. It is mainly produced by natural killer cells and CD4+ and CD8+ T cells, its receptors are found on nearly all cells, where it activates diverse responses that enable potential host cells to prevent invasive infection by bacteria, parasites and viruses. Takayanagi et al. (2000) demonstrated that IFN-γ strongly suppresses osteoclastogenesis by interfering with the RANKL (602642)-RANK (603499) signaling pathway. Tsubota et al. (1999) reported that this upregulation in Sjogren syndrome patients may be controlled by interferon-gamma through the activation of transcription factor NFKB.
Background Reference 1:
Takayanagi, H. et al. Nature 2000. 408: 600-605.
Background Reference 2:
Tsubota, K. et al. Invest. Ophthal. Vis. Sci. 40: 28-34, 1999.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-173

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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