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45-762 (3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
45-762 (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-762 (0.5ug/ml) staining of HepG2 (A) and NIH3T3 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-762 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear sta
45-762 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
45-762 (3ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

IFT88 Antibody

CATALOG NUMBER: 45-762

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, IF, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog
Immunogen:
The immunogen for this antibody is: C-KKRIDEDDFADEE
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, IF, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 85-90kDa band observed in lysates of cell lines HepG2 and NIH3T3 and in Human Kidney lysates (calculated MW of 85.3kDa according to Human NP_001340507.1 and 90.4kDa according to NP_001340498.1, and 93.1kDa according to Mouse NP_033402.2 93. and 89.2kDa according to XP_006518873.1). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of the brush border in PCT. Recommended concentration: 3ug/ml.Immunofluorescence: Expression of the protein seen within the nucleus and cytoplasm of HepG2 cells. Recommended concentration: 10ug/ml. This antibody has been successfully used in IF on Mouse Olfactory and Respiratory Epithelium cells as described in the following paper: Miyoshi et al, FASEB J. 2009 Oct;23(10):3289-97, PMID: 19470799.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Specificity:
This antibody is expected to recognise many reported isoforms.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IFT88
Additional Names:
IFT88, polaris, TTC10, tetratricopeptide repeat domain 10, HGNC:20606, D13S1056E, DAF19, IFT88, MGC26259, RP11-172H24.2, TG737, hTg737, TPR repeat protein 10, Probe hTg737 (polycystic kidney disease, autosomal recessive), Tg737 protein, intraflagellar transport 88 homolog, recessive polycystic kidney disease protein Tg737 homolog, intraflagellar transport 88 homolog (Chlamydomonas)
Protein Accession Number:
NP_783195.2,NP_006522.2,NP_001340507.1,NP_001340498.1
Protein Gi Number:
28558993
Ncbi Gene Id Number:
8100
Background Reference 1:
Vogel G. News focus: Betting on cilia. Science. 2005 Oct 14;310(5746):216-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-762

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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