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Western blot analysis of lysed extracts from 293 cells untreated or treated with insulin using IGF-1R (Phospho-Tyr1165/Tyr1166).
Immunofluorescence staining of methanol-fixed MCF cells using IGF-1R (Phospho-Tyr1165/Tyr1166).
Western blot analysis of lysed extracts from 293 cells untreated or treated with insulin using IGF-1R (Phospho-Tyr1165/Tyr1166).

IGF 1R (phospho Tyr1165 / Tyr1166) Antibody

CATALOG NUMBER: 79-445

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IGF-1R (Phospho-Tyr1165/Tyr1166) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 1165/tyrosine 1166 (T-D-Y (p) -Y (p) -R-K) derived from Human IGF-1R .
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of IGF-1R onlywhen phosphorylated at tyrosine 1165/tyrosine1166.
Predicted Molecular Weight:
200, 95 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
IGF1R
Additional Names:
IGFR, CD221, IGFIR, JTK13, RAB5, Insulin-like growth factor I receptor, IGF1R, kinase IGF1R
Protein Accession Number:
NP_000866.1
Protein Gi Number:
4557665
Ncbi Gene Id Number:
3480
Background:
This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1.
Background Reference 1:
Li S, et al. (1994) J Biol Chem; 269 (51): 32558-64.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-445

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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