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To detect hIGF-I by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human IGF-I (XP-5158) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIGF-I.
To detect hIGF-I by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human IGF-I (XP-5158) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIGF-I.

IGF-I Antibody (biotin)

CATALOG NUMBER: XP-5158Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-I (human IGF-I).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect hIGF-I by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IGF-I (XP-5158) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-I.
Western Blot:
To detect hIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hIGF-I specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
IGF-I antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
IGFBP3
Additional Names:
IBP3, BP-53, IBP1, Insulin-like growth factor I, Mechano growth factor, IGF-I
Protein Accession Number:
P05019-3
Protein Gi Number:
124263
Ncbi Gene Id Number:
3486
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5158Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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