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Western blot analysis of extracts of various cell lines, using IGF2BP1 antibody (15-085) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of K562 cells using 1ug IGF2BP1 antibody (15-085). Western blot was performed from the immunoprecipitate using IGF2BP1 antibody (15-085) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using IGF2BP1 antibody (15-085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

IGF2BP1 Antibody

CATALOG NUMBER: 15-085

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 480 to the C-terminus of human IGF2BP1 (NP_006537.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
Jurkat
Positive Control 2:
K-562
Positive Control 3:
HepG2
Positive Control 4:
HeLa
Positive Control 5:
NIH/3T3
Positive Control 6:
A-549
Predicted Molecular Weight:
Observed: 70kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IGF2BP1
Additional Names:
IGF2BP1, CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1
Ncbi Gene Id Number:
10642
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-085

List Size:
100 uL

List Price:

$436.00

Datasheet
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