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42-596 (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-596 (3.75ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-596 (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

IGF2BP1 Antibody

CATALOG NUMBER: 42-596

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-EKVFAEHKISYSGQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 65kDa band observed in lysates of cell line K562 (calculated MW of 63.5kDa according to NP_006537.3). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Breast. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_006537.3; NP_001153895.1).
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IGF2BP1
Additional Names:
IGF2BP1, insulin-like growth factor 2 mRNA binding protein 1, CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1, IGF II mRNA binding protein 1, IGF2 mRNA-binding protein 1, VICKZ family member 1, coding region determinant-binding protein, zipcode-binding protein 1
Protein Accession Number:
NP_006537.3,NP_001153895.1
Protein Gi Number:
56237027
Ncbi Gene Id Number:
10642

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-596

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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