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Overlay histogram showing HepG2 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: RD whole cell lysate Lane 3: Karpas 299 whole cell lysate Lane 4: Raji whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: Hela whole cell lysate Lane 7: HepG2 whole cell lysate Lane 8: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Overlay histogram showing HepG2 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

IGF2BP1 Antibody

CATALOG NUMBER: 55-194

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Zebrafish, Chicken, Xenopus
Immunogen:
This IGF2BP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 508-534 amino acids from the C-terminal region of human IGF2BP1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
63 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IGF2BP1
Additional Names:
Insulin-like growth factor 2 mRNA-binding protein 1, IGF2 mRNA-binding protein 1, IMP-1, IMP1, Coding region determinant-binding protein, CRD-BP, IGF-II mRNA-binding protein 1, VICKZ family member 1, Zipcode-binding protein 1, ZBP-1, IGF2BP1, CRDBP, VICKZ1, ZBP1
Protein Accession Number:
Q9NZI8
Protein Gi Number:
296434536
Ncbi Gene Id Number:
10642
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IGF2BP1 is a member of the insulin-like growthfactor 2 mRNA-binding protein family. The protein encoded by thisgene contains four K homology domains and two RNA recognitionmotifs. It functions by binding to the mRNAs of certain genes,including insulin-like growth factor 2, beta-actin andbeta-transducin repeat-containing protein, and regulating theirtranslation. Two transcript variants encoding different isoformshave been found for this gene.
Background Reference 1:
Rodriguez, S., et al. Growth Horm. IGF Res. 20(4):310-318(2010)
Background Reference 2:
Pillas, D., et al. PLoS Genet.(2), E1000856 (2010) :
Background Reference 3:
Noubissi, F.K., et al. Cancer Res. 69(22):8572-8578(2009)
Background Reference 4:
Zhou, Y., et al. Virology 393(2):210-220(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-194

List Size:
400 ul

List Price:

$519.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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