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Western blot analysis in HepG2 cell line lysates (35ug/lane).
IGF2BP2 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

IGF2BP2 Antibody

CATALOG NUMBER: 55-046

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This IGF2BP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 530-556 amino acids from the C-terminal region of human IGF2BP2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IGF2BP2
Additional Names:
Insulin-like growth factor 2 mRNA-binding protein 2, IGF2 mRNA-binding protein 2, IMP-2, Hepatocellular carcinoma autoantigen p62, IGF-II mRNA-binding protein 2, VICKZ family member 2, IGF2BP2, IMP2, VICKZ2
Protein Accession Number:
Q9Y6M1
Protein Gi Number:
224471831
Ncbi Gene Id Number:
10644
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the IGF-II mRNA-bindingprotein (IMP) family. The protein encoded by this gene containsseveral four KH domains and two RRM domains. It functions bybinding to the 5' UTR of the insulin-like growth factor 2 (IGF2)mRNA and regulating IGF2 translation. Alternate transcriptionalsplice variants, encoding different isoforms, have beencharacterized.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Pechlivanis, S., et al. Arterioscler. Thromb. Vasc. Biol. 30(9):1867-1872(2010)
Background Reference 3:
Heni, M., et al. Diabetes (2010) In press :
Background Reference 4:
Rodriguez, S., et al. Growth Horm. IGF Res. 20(4):310-318(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-046

List Size:
400 ul

List Price:

$495.00

Datasheet
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