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Western blot analysis of IGFALS Antibody in mouse spleen tissue, Jurkat cell line and mouse liver tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with IGFALS Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of IGFALS Antibody in mouse spleen tissue, Jurkat cell line and mouse liver tissue lysates (35ug/lane)

IGFALS Antibody

CATALOG NUMBER: 62-941

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This IGFALS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-335 amino acids from the Central region of human IGFALS.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
66 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IGFALS
Additional Names:
Insulin-like growth factor-binding protein complex acid labile subunit, ALS, IGFALS, ALS
Protein Accession Number:
P35858
Protein Gi Number:
543800
Ncbi Gene Id Number:
3483
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IGFALS is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this protein are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty.
Background Reference 1:
Leong S.R., Baxter R.C.Mol. Endocrinol. 6:870-876(1992)
Background Reference 2:
Suwanichkul A.Endocrinology 141:833-838(2000)
Background Reference 3:
Liu T., Qian W.-J.J. Proteome Res. 4:2070-2080(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-941

List Size:
400 ul

List Price:

$495.00

Datasheet
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