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43-247 (2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-247 (10ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-247 (2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

IGFBP1 Antibody

CATALOG NUMBER: 43-247

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-STYDGSKALHVTNIK
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in Human Placenta lysates (calculated MW of 27.9kDa according to NP_000587.1). Recommended concentration: 1-3ug/ml.An additional band of 55kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 10ug/ml.
Predicted Molecular Weight:
Approx 30kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IGFBP1
Additional Names:
IGFBP1, insulin-like growth factor binding protein 1, AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IBP-1, IGF-binding protein 1, IGFBP-1, OTTHUMP00000159561, OTTHUMP00000207990, OTTHUMP00000214348, alpha-pregnancy-associated endometrial globulin, amniotic fluid binding protein, binding protein-25, binding protein-26, binding protein-28, growth hormone independent-binding protein, insulin-like growth factor-binding protein 1, placental protein 12
Protein Accession Number:
NP_000587.1
Protein Gi Number:
124055
Ncbi Gene Id Number:
3484
Background Reference 1:
Sugita S, Morishita Y, Kano J, Furuya S, Shiba-Ishii A, Noguchi M.IGFBP-1 is expressed specifically in ovarian clear cell adenocarcinoma.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-247

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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