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Western blot analysis of anti-IGFBP3 Antibody in HepG2 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with IGFBP3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti-IGFBP3 Antibody in HepG2 cell line lysates (35ug/lane).

IGFBP3 Antibody

CATALOG NUMBER: 63-090

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This IGFBP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-193 amino acids from the N-terminal region of human IGFBP3.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
32 kDa
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IGFBP3
Additional Names:
Insulin-like growth factor-binding protein 3, IBP-3, IGF-binding protein 3, IGFBP-3, IGFBP3, IBP3
Protein Accession Number:
P17936
Protein Gi Number:
146327827
Ncbi Gene Id Number:
3486
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IGFBP3 is a member of the insulin-like growth factor binding protein (IGFBP) family with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors.
Background Reference 1:
Muzumdar,R.H., Diabetes 55 (10), 2788-2796 (2006)
Background Reference 2:
Novosyadlyy,R., Growth Horm. IGF Res. 15 (5), 313-323 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-090

List Size:
400 ul

List Price:

$495.00

Datasheet
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