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Western blot analysis of extracts of various cell lines, using IGFBP4 antibody (18-443) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
Immunohistochemistry of paraffin-embedded rat brain using IGFBP4 Antibody (18-443) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using IGFBP4 Antibody (18-443) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IGFBP4 Antibody (18-443) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using IGFBP4 antibody (18-443) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.

IGFBP4 Antibody

CATALOG NUMBER: 18-443

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-258 of human IGFBP4 (NP_001543.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
Positive Control 1:
A-549
Positive Control 2:
SW480
Predicted Molecular Weight:
Observed: 17kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IGFBP4
Additional Names:
IGFBP4, BP-4, HT29-IGFBP, IBP4, IGFBP-4
Ncbi Gene Id Number:
3487
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-443

List Size:
100 uL

List Price:

$436.00

Datasheet
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