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Western blot analysis using IgG monoclonal antibody against human serum IgG.
Western blot analysis using IgG monoclonal antibody against human serum IgG.

IgG Antibody [4D2D9G8]

CATALOG NUMBER: 32-176

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Chrompure Human IgG, Fc Fragment.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Western Blot:1:200 - 1:1,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
IgG monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FCGR2A
Additional Names:
CD32, FCG2, FCGR2A1, IGFR2, Low affinity immunoglobulin gamma Fc region receptor II-a, CDw32, IgG Fc receptor II-a, FcGR, CD32A, FCGR2
Protein Accession Number:
P12318
Protein Gi Number:
160332371
Ncbi Gene Id Number:
2212
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Background Reference 1:
Roitt,I, et al "Immunology", Mosby, London, England, (1996) fourth edition.
Background Reference 2:
Vlug, A. et al "The structure and function of human IgG subclasses". Eur.Clin.Lab. 8, 26 (1989).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-176

List Size:
0.1 mg

List Price:

$302.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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