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The purity of Mouse IgG2a Fc Region was determined by DTT-reduced (+) and non-reduced (-) SDS-PAGE and staining overnight with Coomassie Blue.
The purity of Mouse IgG2a Fc Region was determined by DTT-reduced (+) and non-reduced (-) SDS-PAGE and staining overnight with Coomassie Blue.

IgG2a Fc Region Recombinant Protein

CATALOG NUMBER: 96-421

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Mouse
source-species:
HEK293 cells
Recombinant Protein Sequence:
Glu 98 - Lys 330
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
26.4 kDa
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
50 mM tris, 100 mM glycine, pH7.5
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Ighg2a
Protein Accession Number:
CAC20702
Ncbi Gene Id Number:
380793
Background:
Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.
Background Reference 1:
Sikorav J.-L., et al., 1980, Nucleic Acids Res. 8:3143-3155.
Background Reference 2:
Ollo R., et al., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:2442-2446.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-421

List Size:
0.2 mg

List Price:

$495.00

Datasheet
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