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Western blot analysis of extracts of various cell lines, using IGHMBP2 antibody (22-806) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using IGHMBP2 antibody (22-806) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

IGHMBP2 Antibody

CATALOG NUMBER: 22-806

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of mouse IGHMBP2
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
Jurkat
Positive Control 2:
MCF7
Positive Control 3:
Mouse brain
Positive Control 4:
Rat brain
Positive Control 5:
Rat liver
Predicted Molecular Weight:
Observed: 109kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IGHMBP2
Additional Names:
AEP, Catf1, RIPE3b1, Smbp-2, Smbp2, Smubp2, nmd, sma, DNA-binding protein SMUBP-2, ATP-dependent helicase IGHMBP2, antifreeze enhancer-binding protein, cardiac transcription factor 1, immunoglobulin S mu binding protein 2, neuromuscular degeneration, p110 subunit
Ncbi Gene Id Number:
20589
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a helicase superfamily member that binds a specific DNA sequence from the immunoglobulin mu chain switch region. Mutations in this gene lead to spinal muscle atrophy with respiratory distress type 1.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-806

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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