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Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).
IGSF8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ug per lane.

IGSF8 Antibody

CATALOG NUMBER: 56-390

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This IGSF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-254 amino acids from the Central region of human IGSF8.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IGSF8
Additional Names:
Immunoglobulin superfamily member 8, IgSF8, CD81 partner 3, Glu-Trp-Ile EWI motif-containing protein 2, EWI-2, Keratinocytes-associated transmembrane protein 4, KCT-4, LIR-D1, Prostaglandin regulatory-like protein, PGRL, CD316, IGSF8, CD81P3, EWI2, KCT4
Protein Accession Number:
Q969P0
Protein Gi Number:
74762642
Ncbi Gene Id Number:
93185
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IGSF8 may play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.
Background Reference 1:
Kolesnikova, T.V., et al. Neoplasia 11(1):77-86(2009)
Background Reference 2:
Rocha-Perugini, V., et al. PLoS ONE(4), E1866 (2008) :
Background Reference 3:
Kettner, S., et al. Mol. Cell. Biol. 27(21):7718-7726(2007)
Background Reference 4:
Sala-Valdes, M., et al. J. Biol. Chem. 281(28):19665-19675(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-390

List Size:
400 ul

List Price:

$495.00

Datasheet
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