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Western blot analysis in HepG2 cell line lysates (35ug/lane).
IHH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in HepG2 cell line lysates (35ug/lane).

IHH Antibody

CATALOG NUMBER: 58-107

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This IHH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-80 amino acids from the N-terminal region of human IHH.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IHH
Additional Names:
Indian hedgehog protein, IHH, HHG-2, Indian hedgehog protein N-product, Indian hedgehog protein C-product, IHH
Protein Accession Number:
Q14623
Protein Gi Number:
33112634
Ncbi Gene Id Number:
3549
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the hedgehog family ofsecreted signaling molecules. Hedgehog proteins are essentialregulators of a variety of developmental processes includinggrowth, patterning and morphogenesis. The encoded proteinspecifically plays a role in bone growth an differentiation.Mutations in this gene are the cause of brachydactyly type A1 whichis characterized by shortening or malformation of the phalanges.Mutations in this gene are also the cause of acrocapitofemoraldysplasia.
Background Reference 1:
Meulenbelt, I., et al. Ann. Rheum. Dis. (2010) In press :
Background Reference 2:
Kang, S.J., et al. Hum. Mol. Genet. 19(13):2725-2738(2010)
Background Reference 3:
Okada, Y., et al. Hum. Mol. Genet. 19(11):2303-2312(2010)
Background Reference 4:
Zhao, J., et al. BMC Med. Genet. 11, 96 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-107

List Size:
400 ul

List Price:

$495.00

Datasheet
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