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Western blot analysis of lysed extracts from 293 cells untreated or treated with TNF using IkB-ε (Phospho-Ser22).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-ε (Phospho-Ser22).
Western blot analysis of lysed extracts from 293 cells untreated or treated with TNF using IkB-ε (Phospho-Ser22).

IkB epsilon (phospho Ser22) Antibody

CATALOG NUMBER: 79-197

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
IkB-epsilon (Phospho-Ser22) antibody was raised against a peptide sequence around phosphorylation site of serine 22 (I-E-S (p) -L-R) derived from Human IkB-ε.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of IkappaB-ε only when phosphorylated at serine 22.
Predicted Molecular Weight:
40 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
NFKBIE
Additional Names:
IKBE, BHLHE39, I-kappa-B-epsilon, IKBE, IkappaBepsilon, NF-kappa-BIE, NF-kappaB inhibitor epsilon
Protein Accession Number:
NP_004547.2
Protein Gi Number:
71274109
Ncbi Gene Id Number:
4794
Background:
Inhibits NF-κ-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-κ-B p50-p65 and p50-c-Rel complexes.
Background Reference 1:
Shirane M, et al. (1999) J Biol Chem; 274 (40): 28169-74Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663. Chen, Z. J. et al. (1996) Cell; 84, 853-862. Brown, K. et al. (1995) Science; 267, 1485-1488.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-197

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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