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Western blot analysis of lysed extracts from 293 cells untreated or treated with TNF using IKK α (Phospho-Thr23).
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using IKK α (Phospho-Thr23).
Western blot analysis of lysed extracts from 293 cells untreated or treated with TNF using IKK α (Phospho-Thr23).

IKK a (phospho Thr23) Antibody

CATALOG NUMBER: 79-161

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IKK a (Phospho-Thr23) antibody was raised against a peptide sequence around phosphorylation site of threonine 23 (L-G-T (p) -G-G) derived from Human IKK α.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity:
This antibody detects endogenous level of IKKα only when phosphorylated at threonine 23.
Predicted Molecular Weight:
85 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CHUK
Additional Names:
IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, CTNNB, OK/SW-cl.35, PRO2286, I kappa-B kinase alpha, I-kappa-B kinase 1, IKK-A, IKK1
Protein Accession Number:
NP_001269.3
Protein Gi Number:
62241001
Ncbi Gene Id Number:
1147
Background:
Acts as part of the IKK complex in the conventional pathway of NF-κ-B activation and phosphorylates inhibitors of NF-κ-B thus leading to the dissociation of the inhibitor/NF-κ-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-κ-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-κ-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-κ-B-regulated promoters during inflammatory responses triggered by cytokines.
Background Reference 1:
Yuan ZQ, et al. (2002) J Biol Chem; 277 (33): 29973-82. Ozes ON, et al. (1999) Nature; 401 (6748): 82-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-161

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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