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Western blot analysis of extracts of various cell lines, using IKK alpha antibody (18-473) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using IKK alpha antibody (18-473) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using IKK alpha antibody (18-473) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using IKK alpha antibody (18-473). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug IKK alpha antibody (18-473). Western blot was performed from the immunoprecipitate using IKK alpha antibody (18-473) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using IKK alpha antibody (18-473) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

IKK alpha Antibody

CATALOG NUMBER: 18-473

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 650 to the C-terminus of human IKK alpha (NP_001269.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
NIH/3T3
Positive Control 3:
HT-29
Predicted Molecular Weight:
Observed: 94kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CHUK
Additional Names:
IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Inhibitor of nuclear factor kappa-B kinase subunit alpha, Conserved helix-loop-helix ubiquitous kinase, I-kappa-B kinase alpha
Ncbi Gene Id Number:
1147
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-473

List Size:
100 uL

List Price:

$436.00

Datasheet
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