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Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from NIH-3T3 cells treated with TNF using IKK- alpha/ beta (Phospho-Ser176/177) Antibody. The lane on the right is treated with the antigen-specific peptide.

IKK- alpha/ beta (phospho Ser176/177) Antibody

CATALOG NUMBER: 79-982

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IKK- alpha/ beta (phospho Ser176/177) antibody was raised against a peptide sequence around phosphorylation site of serine 176/177 (Q-G-S (p)-L-C) derived from Human IKK-alpha/beta.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous level of IKK- alpha/beta only when phosphorylated at serine 176/177.
Predicted Molecular Weight:
85 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
CHUK
Additional Names:
FLJ40509, I-kappa-B kinase, IKBKB, kinase beta, NFKBIKB
Protein Accession Number:
NP_001269.3
Protein Gi Number:
62241001
Ncbi Gene Id Number:
1147
Background:
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3.
Background Reference 1:
Chandrakesan P, et al. (2010)J Biol Chem 285, 33485-98
Background Reference 2:
Hinz M, et al. (2010 Mol Cell 40, 63-74
Background Reference 3:
Choudhary S, Lu M, Cui R, Brasier AR (2007)Mol Endocrinol 21, 2203-17

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-982

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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