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Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/mL.
Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 μg/mL.
Immunofluorescence of IKK gamma in Hela cells with IKK gamma antibody at 20 μg/mL.<br><br>Red: IKK gamma Antibody (2335) <br> Blue: DAPI staining
Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/mL.
Green Product
Trial Size

IKK gamma Antibody

CATALOG NUMBER: 2335

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
IKK gamma antibody was raised against a 17 amino acid peptide near the carboxy terminus of human IKK gamma.
The immunogen is located within the last 50 amino acids of IKK gamma.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
IKK gamma antibody can be used for detection of IKK gamma by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
IKK gamma has no cross response to IKK alpha or IKK beta.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
52 kDa
Purification:
IKK gamma Antibody is DEAE purified.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
IKK gamma Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
IKK gamma antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IKBKG
Additional Names:
IKK gamma Antibody: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, ZC2HC9, IKK-gamma, NF-kappa-B essential modulator
Protein Accession Number:
AF074382
Protein Gi Number:
3641279
Ncbi Gene Id Number:
8517
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IKK gamma Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The IκB kinase (IKKα and IKKβ) phosphorylates IkB and mediates NF-κB activation. A novel molecule in the IKK complex was recently identified and termed IKKγ/NEMO/FIP3/IKKAP1. IKKγ interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation.
Background Reference 1:
Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-γ is an essential regulatory subunit of the IκB kinase complex. Nature 1998;395(6699):297-300
Background Reference 2:
Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou F, Kirk HE, Kay RJ, Israel A. Complementation cloning of NEMO, a component of the IκB kinase complex essential for NF-κB activation. Cell. 1998;93(7):1231-40.
Background Reference 3:
Li Y, Kang J, Friedman J, Tarassishin L, Ye J, Kovalenko A, Wallach D, Horwitz MS. Identification of a cell protein (FIP-3) as a modulator of NF-κB activity and as a target of an adenovirus inhibitor of tumor necrosis factor α-induced apoptosis. Proc Natl Acad Sci U S A 1999;96(3):1042-7
Background Reference 4:
Mercurio F, Murray BW, Shevchenko A, Bennett BL, Young DB, Li JW, Pascual G, Motiwala A, Zhu H, Mann M, Manning AM. IκB kinase (IKK)-associated protein 1, a common component of the heterogeneous IKK complex. Mol Cell Biol. 1999;19(2):1526-38.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2335

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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