Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/mL.
Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 μg/mL.
Immunofluorescence of IKK gamma in Hela cells with IKK gamma antibody at 20 μg/mL.<br><br>Red: IKK gamma Antibody (2335) <br> Blue: DAPI staining
Western blot analysis of IKK gamma in HeLa whole cell lysate with IKK gamma antibody at 1 μg/mL.
Trial Size

IKK gamma Antibody

CATALOG NUMBER: 2335

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
IKK gamma antibody was raised against a 17 amino acid peptide near the carboxy terminus of human IKK gamma.
The immunogen is located within the last 50 amino acids of IKK gamma.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
IKK gamma antibody can be used for detection of IKK gamma by Western blot at 1 μg/mL. A 52 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
IKK gamma has no cross response to IKK alpha or IKK beta.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
52 kDa
Purification:
IKK gamma Antibody is DEAE purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IKK gamma Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
IKK gamma antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IKBKG
Additional Names:
IKK gamma Antibody: IP, IP1, IP2, FIP3, IPD2, NEMO, FIP-3, Fip3p, AMCBX1, ZC2HC9, IKK-gamma, NF-kappa-B essential modulator
Protein Accession Number:
AF074382
Protein Gi Number:
3641279
Ncbi Gene Id Number:
8517
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IKK gamma Antibody: Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. The IκB kinase (IKKα and IKKβ) phosphorylates IkB and mediates NF-κB activation. A novel molecule in the IKK complex was recently identified and termed IKKγ/NEMO/FIP3/IKKAP1. IKKγ interacts with IKKβ and is required for the activation of IKK complex and NF-κB by LPS, PMA, TNF, and IL-1 stimulation. FIP3 was also shown to bind to RIP and NIK and to mediate TNF-induced NF-κB activation.
Background Reference 1:
Rothwarf DM, Zandi E, Natoli G, Karin M. IKK-γ is an essential regulatory subunit of the IκB kinase complex. Nature 1998;395(6699):297-300
Background Reference 2:
Yamaoka S, Courtois G, Bessia C, Whiteside ST, Weil R, Agou F, Kirk HE, Kay RJ, Israel A. Complementation cloning of NEMO, a component of the IκB kinase complex essential for NF-κB activation. Cell. 1998;93(7):1231-40.
Background Reference 3:
Li Y, Kang J, Friedman J, Tarassishin L, Ye J, Kovalenko A, Wallach D, Horwitz MS. Identification of a cell protein (FIP-3) as a modulator of NF-κB activity and as a target of an adenovirus inhibitor of tumor necrosis factor α-induced apoptosis. Proc Natl Acad Sci U S A 1999;96(3):1042-7
Background Reference 4:
Mercurio F, Murray BW, Shevchenko A, Bennett BL, Young DB, Li JW, Pascual G, Motiwala A, Zhu H, Mann M, Manning AM. IκB kinase (IKK)-associated protein 1, a common component of the heterogeneous IKK complex. Mol Cell Biol. 1999;19(2):1526-38.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2335

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369