Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of IKZF2 in EL4 cell lysate with IKZF2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of IKZF2 in human spleen tissue with IKZF2 antibody at 5 μg/mL.
Immunofluorescence of IKZF2 in human spleen tissue with IKZF2 antibody at 20 μg/mL.
Western blot analysis of IKZF2 in EL4 cell lysate with IKZF2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

IKZF2 Antibody

CATALOG NUMBER: 8117

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IKZF2 antibody was raised against a 19 amino acid peptide near the amino terminus of human IKZF2.
The immunogen is located within amino acids 50 - 100 of IKZF2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
IKZF2 antibody can be used for detection of IKZF2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
IKZF2 antibody is human, mouse and rat reactive. At least two isoforms of IKZF2 are known to exist; this antibody will detect both isoforms. IKZF2 antibody is predicted to not cross-react with other IKZF proteins.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 58 kDa
Observed: 60 kDa
Purification:
IKZF2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IKZF2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IKZF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
IKZF2
Additional Names:
Ikaros family zinc finger 2 (Helios), ANF1A2, HELIOS, ZNF1A2, ZNFN1A2
Protein Accession Number:
NP_057344
Protein Gi Number:
119220596
Ncbi Gene Id Number:
22807
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Ikaros family zinc finger 2 (IKZF2) is a member of the Ikaros family of zinc-finger proteins, a family of hematopoietic-specific transcription factors involved in the regulation of lymphocyte development (1,2). IKZF2 forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development (3). Its expression is a marker for T cell activation and proliferation (4).
Background Reference 1:
Hahm K, Cobb BS, McCarty AS, et al. Helios, a T cell-restricted Ikaros family member that quantitatively associates with Ikaros at centromeric heterochromatin. Genes Dev. 1998; 12:782-96.
Background Reference 2:
John LB and Ward AC. The Ikaros gene family: transcriptional regulators of hematopoiesis and immunity. Mol. Immunol. 2011; 48:1272-8.
Background Reference 3:
Kelly CM, Ikeda T, Koipally J, et al. Helios, a novel dimerization partner of Ikaros expressed in the earliest hematopoietic progenitors. Curr. Biol. 1998; 8:508-15.
Background Reference 4:
Akimova T, Beier UH, Wang L, et al. Helios expression is a marker for T cell activation and proliferation. PLoS One 2011; 6:e24226.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8117

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369